Enamel Thickness Determination by Optical Coherence Tomography: In vitro Validation
Abstract Optical coherence tomography (OCT) has been introduced in dentistry as a nondestructive diagnostic imaging tool that does not utilize ionizing radiation. This study investigated the agreement between polarization-sensitive OCT (PSOCT), microcomputed tomography (μ-CT), and histology for enamel thickness measurements. Human enamel samples were prepared and evaluated with μ-CT and PS-OCT and then sectioned and observed via digital transversal light microscopy. For all methods, a standard transversal section (b-scan) in each sample was selected, and the enamel thickness was measured at three predetermined positions using ImageJ analysis software.