Profilometric Quantification of Erosive Tissue Loss in Dentine: A Systematic Evaluation of the Method
Profilometry is established in erosion research. However, in the case of dentine, factors such as the demineralised organic matrix, desiccation effects, or type of measuring device may have an impact on the measurement results, which were investigated in the present study. Dentine specimens were eroded with citric acid (1%, pH 2.6) for 5, 10, 15, 20, 30, 60, 90, and 120 min (n = 15 each). For each specimen, tissue loss was determined under various conditions – before/after enzymatic matrix removal, under standardised wet and desiccated (2/10 min) conditions – with non-contact and contact profilometry. In the presence of matrix, under wet conditions, non-contact profilometry revealed almost no tissue loss. Values (mean ± SD) ranged between 0.3 ± 0.7 μm (5 min) and 3.4 ± 1.5 μm (120 min).